RICE SCIENCE ›› 2009, Vol. 16 ›› Issue (4): 247-253 .DOI: 10.1016/S1672-6308(08)60087-6

• 研究报告 •    下一篇

Rapid Construction of a High-Density Rice Linkage Map by High Efficiency Genome Scanning (HEGS) System

Akifumi SHIMIZU1, 2, Shinji KAWASAKI1   

  1. 1 National Institute of Agrobiological Sciences, Kannon-dai, Tsukuba, Ibaraki, 305-8602, Japan; 2 Present address: School of Environmental Science, University of Shiga Prefecture, Hassaka, Hikone, Shiga 522-8533, Japan
  • 收稿日期:2009-06-09 出版日期:2009-12-28 发布日期:2009-12-28
  • 通讯作者: Akifumi SHIMIZU
  • 基金资助:
    the funds from CREST (Core research for evolutional science and technology) of the Japan Science and Technology Corporation, and BRAIN, Bio-oriented Technology Research Advancement Institution, Japan.

摘要: High-density linkage maps are essential tools for genome analysis of various biological traits. Our developed compact multi-gel system, HEGS (high efficiency genome scanning) is a high-throughput and high-cost-performance electrophoresis apparatus. Using this system, a high-density (average interval 2.3 cM) map with 1 065 AFLP and 63 SSR markers was constructed from recombinant inbred lines of a japonica and indica hybrid in just two months of electrophoreses by a single person. More than 50% of the mapped AFLP markers were commonly polymorphic for several combinations between japonica and indica rice and 15% were applicable for genetically closer crosses between upland and lowland types of japonica rice. This system can be used for rapid analyses of all kinds of markers.

关键词: rice, high-density linkage map, high efficiency genome scanning system